When Krsna left Vrndavana for Mathura, the gopis became most dejected and spent the rest of their lives simply crying in separation from Krsna. This means that in one sense they were never actually separated from Krsna. There is no difference between thinking of Krsna and associating with Him. Rather, vipralambha-seva, thinking of Krsna in separation, as Sri Caitanya Mahaprabhu did, is far better than serving Krsna directly.
Thus of all the devotees who have developed unalloyed devotional love for Krsna, the gopis are most exalted, and out of all these exalted gopis, Srimati Radharani is the highest. No one can excel the devotional service of Srimati Radharani.
The gopis of Vrndavana were so attached to Krsna that they were not satisfied simply with the rasa dance at night. They wanted to associate with Him and enjoy His company during the daytime also. When Krsna went to the forest with His cowherd boyfriends and cows, the gopis did not physically take part, but their hearts went with Him. And because their hearts went, they were able to enjoy His company through strong feelings of separation. To acquire this strong feeling of separation is the teaching of Lord Caitanya and His direct disciplic succession of Gosvamis. When we are not in physical contact with Krsna, we can associate with Him like the gopis, through feelings of separation. Krsna’s transcendental form, qualities, pastimes and entourage are all identical with Him. There are nine different kinds of devotional service.
Devotional service to Krsna in feelings of separation elevates the devotee to the highest perfectional level, to the level of the gopis.
Those who are in the disciplic succession of the Madhva-Gaudiya-sampradaya should also feel the separation of Krsna, worship His transcendental form, and discuss His transcendental teachings, His pastimes, His qualities, His entourage and His associations. The spiritual masters should enrich the devotees to the highest devotional perfection. Feeling constant separation while engaged in the service of the Lord is the perfection of Krsna consciousness.
Selections from Srila Prabhupada’s Krsna Book, The Gopis Feelings of Separation